| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Verrucomicrobium spinosum | TTTGTGCTGCGCAACACC | CAGGCGATCACCAAAGCAG | 59.90 | 59.20 | 207 | 145 |
100.00%
|
100.00%
|
| Primer 2 | Verrucomicrobium spinosum | ACGCCAGCAGAATCTGTGG | TTCACCGTCGAAACCCAGG | 60.38 | 59.93 | 200 | 139 |
100.00%
|
100.00%
|
| Primer 3 | Verrucomicrobium spinosum | TTTGTGCTGCGCAACACC | ATCACCAAAGCAGCGCAC | 59.90 | 59.04 | 201 | 133 |
100.00%
|
100.00%
|
| Primer 4 | Verrucomicrobium spinosum | TTTGTGCTGCGCAACACC | GATCACCAAAGCAGCGCAC | 59.90 | 60.15 | 202 | 133 |
100.00%
|
100.00%
|
| Primer 5 | Verrucomicrobium spinosum | AGCAGTTTCCAAGCGGTTC | GCATGTTGGCCTTGACTGC | 58.67 | 60.08 | 164 | 125 |
100.00%
|
100.00%
|
| Primer 6 | Verrucomicrobium spinosum | TTGCTCTTGGCGTGTTGC | TGAAGCTCCCATGTGCCAG | 59.28 | 60.00 | 188 | 118 |
100.00%
|
100.00%
|
| Primer 7 | Verrucomicrobium spinosum | AACGTCACATGCCTGGAGG | ACCACACACCGCATACCTG | 60.00 | 60.00 | 164 | 114 |
100.00%
|
100.00%
|
| Primer 8 | Verrucomicrobium spinosum | ACCCGTGAGCAATTCGAGG | ACGGCAACTCACCTGCAT | 60.08 | 59.57 | 256 | 113 |
100.00%
|
100.00%
|
| Primer 9 | Verrucomicrobium spinosum | CTCCGCTTCATGCACAAGC | ACGGCAACTCACCTGCAT | 59.86 | 59.57 | 232 | 113 |
100.00%
|
100.00%
|
| Primer 10 | Verrucomicrobium spinosum | TCCGCTTCATGCACAAGC | ACGGCAACTCACCTGCAT | 58.73 | 59.57 | 231 | 113 |
100.00%
|
100.00%
|